Jessica Chastain successfully spanked Madonna during her Rebel Heart tour stop at the O2 Arena in Prague, Czech Republic on Saturday, Nov. 7.

The 38-year-old "Zero Dark Thirty" actress jumped on stage to smack the singer's behind during her song "Unapologetic B*tch," according to the Daily Mail.

After getting down on her hands and knees, Madonna was slapped on her rump four consecutive times by the actress. Chastain posted a video on her Facebook of the incident, captioning the video, "I gave Madonna a spanking & Ive [sic] a video to prove it. #rebelheart #unapologeticb****."

During her appearance, Chastain donned a sheer long-sleeved top, which revealed a lacy bra underneath, black trousers and boots. While on stage the red headed actress was also seen grinding alongside the 57-year-old singer's backup dancers.

The actress also posted a photo to her Instagram of her holding hands with Madonna, with the caption, "The moment right before @madonna handed me a banana. The fkn Queen. #rebelheart #unapologeticb****."

 The moment right before @madonna handed me a banana. The fkn QUEEN. #rebelheart #unapologeticbitch

A photo posted by Jessica Chastain (@chastainiac) on Nov 7, 2015 at 4:55pm PST

Madonna also shared the photo with 5 million followers on Instagram and her own caption, which read, "Tonights [sic] sexy and beautiful unapologetic b****. Thank you Jessica Chastain!! Thank you Prague! #rebelhearttour."

Once the song ended the actress and singer hugged and Madonna asked Chastain if she had fun performing on stage. Chastain responded, "Yes? You're a very good b****. Right guys?" while looking back at the singer's male dancers.

Chastain currently stars in the drama horror film "Crimson Peak," which was released to theaters October 16. The film, directed by Guillermo del Toro, also stars Mia Wasikowska and Tom Hiddleston.

Madonna's Rebel Heart tour will continue to make its way through Europe, Asia and Oceania, wrapping up on March 27, 2016.

The singer released her thirteenth studio album, "Rebel Heart," on March 6. The album, which peaked at No. 2 on the U.S. Billboard 200 chart, has produced the singles "Living for Love," "Ghosttown" and "Bitch I'm Madonna."